ot anaspec Search Results


pro 8 -ot  (AnaSpec)
90
AnaSpec pro 8 -ot
Potency of Leu 8 -OT and <t> Pro 8 -OT </t> at inducing calcium mobilization in mOTR and hOTR CHO cells
Pro 8 Ot, supplied by AnaSpec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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leu 8 -ot  (AnaSpec)
90
AnaSpec leu 8 -ot
Representative primate species OTRs
Leu 8 Ot, supplied by AnaSpec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/leu 8 -ot/product/AnaSpec
Average 90 stars, based on 1 article reviews
leu 8 -ot - by Bioz Stars, 2026-02
90/100 stars
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ova peptide sinfekl  (AnaSpec)
90
AnaSpec ova peptide sinfekl
IEC of Rnf5 −/− mice activate immune response and change anti-microbial peptide (AMP) expression. a Villi length and crypt depth calculated from H&E-stained sections of intestines from WT or Rnf5 −/− mice (WT, n = 30; Rnf5 −/− , n = 32). b qRT-PCR analysis of AMPs mRNA levels in IECs from small intestine of naive WT or Rnf5 −/− mice ( n = 6). c Representative images (left) and quantification (right) of cleaved caspase-3 immunostained small intestine organoids from tumor-bearing WT or Rnf5 −/− mice ( n = 3). Scale bar = 100μm. Graph shows percentage of cleaved caspase-3 + cells per immunostained organoid ( n = 12 fields). d Intracellular IFN-γ and TNF-α staining of p14 CD8 + T cells incubated for 72 h with 2 μg/ml GP33 peptide recognized by the TCR of P14 and bone marrow-derived dendritic cells (BMDCs) that were incubated with medium alone (no stimulation) or with conditioned medium (CM) from shControl or shRNF5 MODE-K cells. e Representative images (left) and quantification (right) of CD11c + cell immunostaining in the small intestine of WT or Rnf5 −/− mice on day 24 after injection of YUMM1.5 cells. Scale bar = 50μm ( n = 4). f Frequencies of total DCs and pDCs in Peyer’s patches from WT and Rnf5 −/− mice on day 10 after YUMM1.5 cell injection ( n = 6). g 10 days after tumor injection, DCs from GALT, dLN, and ndLN were isolated, pooled per group ( n = 10 mice/group), and were incubated with OT-1 CD8 + T cells stimulated with 2 μg/ml <t>OVA</t> peptide <t>(SINFEKL).</t> Intracellular IFN-γ and TNF-α of OT-1 CD8 + T cells were detected. Data are representative of three independent experiments ( b , d ) and two independent experiments ( a , c , e , f , g ) ≥3 mice per group. Graphs show the mean ± s.e.m. * P < 0.05, ** P < 0.005, *** P < 0.001, **** P < 0.0001 by two-tailed t test or Mann–Whitney U test ( a , b , c , e , f ) or one-way ANOVA with Tukey’s ( d ) correction for multiple comparisons
Ova Peptide Sinfekl, supplied by AnaSpec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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ovalbumin peptide 332–339 ot-ii peptide  (AnaSpec)
90
AnaSpec ovalbumin peptide 332–339 ot-ii peptide
IEC of Rnf5 −/− mice activate immune response and change anti-microbial peptide (AMP) expression. a Villi length and crypt depth calculated from H&E-stained sections of intestines from WT or Rnf5 −/− mice (WT, n = 30; Rnf5 −/− , n = 32). b qRT-PCR analysis of AMPs mRNA levels in IECs from small intestine of naive WT or Rnf5 −/− mice ( n = 6). c Representative images (left) and quantification (right) of cleaved caspase-3 immunostained small intestine organoids from tumor-bearing WT or Rnf5 −/− mice ( n = 3). Scale bar = 100μm. Graph shows percentage of cleaved caspase-3 + cells per immunostained organoid ( n = 12 fields). d Intracellular IFN-γ and TNF-α staining of p14 CD8 + T cells incubated for 72 h with 2 μg/ml GP33 peptide recognized by the TCR of P14 and bone marrow-derived dendritic cells (BMDCs) that were incubated with medium alone (no stimulation) or with conditioned medium (CM) from shControl or shRNF5 MODE-K cells. e Representative images (left) and quantification (right) of CD11c + cell immunostaining in the small intestine of WT or Rnf5 −/− mice on day 24 after injection of YUMM1.5 cells. Scale bar = 50μm ( n = 4). f Frequencies of total DCs and pDCs in Peyer’s patches from WT and Rnf5 −/− mice on day 10 after YUMM1.5 cell injection ( n = 6). g 10 days after tumor injection, DCs from GALT, dLN, and ndLN were isolated, pooled per group ( n = 10 mice/group), and were incubated with OT-1 CD8 + T cells stimulated with 2 μg/ml <t>OVA</t> peptide <t>(SINFEKL).</t> Intracellular IFN-γ and TNF-α of OT-1 CD8 + T cells were detected. Data are representative of three independent experiments ( b , d ) and two independent experiments ( a , c , e , f , g ) ≥3 mice per group. Graphs show the mean ± s.e.m. * P < 0.05, ** P < 0.005, *** P < 0.001, **** P < 0.0001 by two-tailed t test or Mann–Whitney U test ( a , b , c , e , f ) or one-way ANOVA with Tukey’s ( d ) correction for multiple comparisons
Ovalbumin Peptide 332–339 Ot Ii Peptide, supplied by AnaSpec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
ovalbumin peptide 332–339 ot-ii peptide - by Bioz Stars, 2026-02
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ot-ii peptide (chicken ova amino acids 323-339  (AnaSpec)
90
AnaSpec ot-ii peptide (chicken ova amino acids 323-339
IEC of Rnf5 −/− mice activate immune response and change anti-microbial peptide (AMP) expression. a Villi length and crypt depth calculated from H&E-stained sections of intestines from WT or Rnf5 −/− mice (WT, n = 30; Rnf5 −/− , n = 32). b qRT-PCR analysis of AMPs mRNA levels in IECs from small intestine of naive WT or Rnf5 −/− mice ( n = 6). c Representative images (left) and quantification (right) of cleaved caspase-3 immunostained small intestine organoids from tumor-bearing WT or Rnf5 −/− mice ( n = 3). Scale bar = 100μm. Graph shows percentage of cleaved caspase-3 + cells per immunostained organoid ( n = 12 fields). d Intracellular IFN-γ and TNF-α staining of p14 CD8 + T cells incubated for 72 h with 2 μg/ml GP33 peptide recognized by the TCR of P14 and bone marrow-derived dendritic cells (BMDCs) that were incubated with medium alone (no stimulation) or with conditioned medium (CM) from shControl or shRNF5 MODE-K cells. e Representative images (left) and quantification (right) of CD11c + cell immunostaining in the small intestine of WT or Rnf5 −/− mice on day 24 after injection of YUMM1.5 cells. Scale bar = 50μm ( n = 4). f Frequencies of total DCs and pDCs in Peyer’s patches from WT and Rnf5 −/− mice on day 10 after YUMM1.5 cell injection ( n = 6). g 10 days after tumor injection, DCs from GALT, dLN, and ndLN were isolated, pooled per group ( n = 10 mice/group), and were incubated with OT-1 CD8 + T cells stimulated with 2 μg/ml <t>OVA</t> peptide <t>(SINFEKL).</t> Intracellular IFN-γ and TNF-α of OT-1 CD8 + T cells were detected. Data are representative of three independent experiments ( b , d ) and two independent experiments ( a , c , e , f , g ) ≥3 mice per group. Graphs show the mean ± s.e.m. * P < 0.05, ** P < 0.005, *** P < 0.001, **** P < 0.0001 by two-tailed t test or Mann–Whitney U test ( a , b , c , e , f ) or one-way ANOVA with Tukey’s ( d ) correction for multiple comparisons
Ot Ii Peptide (Chicken Ova Amino Acids 323 339, supplied by AnaSpec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ot-ii peptide (chicken ova amino acids 323-339/product/AnaSpec
Average 90 stars, based on 1 article reviews
ot-ii peptide (chicken ova amino acids 323-339 - by Bioz Stars, 2026-02
90/100 stars
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ot-ii ova peptide  (AnaSpec)
90
AnaSpec ot-ii ova peptide
IEC of Rnf5 −/− mice activate immune response and change anti-microbial peptide (AMP) expression. a Villi length and crypt depth calculated from H&E-stained sections of intestines from WT or Rnf5 −/− mice (WT, n = 30; Rnf5 −/− , n = 32). b qRT-PCR analysis of AMPs mRNA levels in IECs from small intestine of naive WT or Rnf5 −/− mice ( n = 6). c Representative images (left) and quantification (right) of cleaved caspase-3 immunostained small intestine organoids from tumor-bearing WT or Rnf5 −/− mice ( n = 3). Scale bar = 100μm. Graph shows percentage of cleaved caspase-3 + cells per immunostained organoid ( n = 12 fields). d Intracellular IFN-γ and TNF-α staining of p14 CD8 + T cells incubated for 72 h with 2 μg/ml GP33 peptide recognized by the TCR of P14 and bone marrow-derived dendritic cells (BMDCs) that were incubated with medium alone (no stimulation) or with conditioned medium (CM) from shControl or shRNF5 MODE-K cells. e Representative images (left) and quantification (right) of CD11c + cell immunostaining in the small intestine of WT or Rnf5 −/− mice on day 24 after injection of YUMM1.5 cells. Scale bar = 50μm ( n = 4). f Frequencies of total DCs and pDCs in Peyer’s patches from WT and Rnf5 −/− mice on day 10 after YUMM1.5 cell injection ( n = 6). g 10 days after tumor injection, DCs from GALT, dLN, and ndLN were isolated, pooled per group ( n = 10 mice/group), and were incubated with OT-1 CD8 + T cells stimulated with 2 μg/ml <t>OVA</t> peptide <t>(SINFEKL).</t> Intracellular IFN-γ and TNF-α of OT-1 CD8 + T cells were detected. Data are representative of three independent experiments ( b , d ) and two independent experiments ( a , c , e , f , g ) ≥3 mice per group. Graphs show the mean ± s.e.m. * P < 0.05, ** P < 0.005, *** P < 0.001, **** P < 0.0001 by two-tailed t test or Mann–Whitney U test ( a , b , c , e , f ) or one-way ANOVA with Tukey’s ( d ) correction for multiple comparisons
Ot Ii Ova Peptide, supplied by AnaSpec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ot-ii ova peptide/product/AnaSpec
Average 90 stars, based on 1 article reviews
ot-ii ova peptide - by Bioz Stars, 2026-02
90/100 stars
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biotin-labeled ot (anaspec inc)  (AnaSpec)
90
AnaSpec biotin-labeled ot (anaspec inc)
IEC of Rnf5 −/− mice activate immune response and change anti-microbial peptide (AMP) expression. a Villi length and crypt depth calculated from H&E-stained sections of intestines from WT or Rnf5 −/− mice (WT, n = 30; Rnf5 −/− , n = 32). b qRT-PCR analysis of AMPs mRNA levels in IECs from small intestine of naive WT or Rnf5 −/− mice ( n = 6). c Representative images (left) and quantification (right) of cleaved caspase-3 immunostained small intestine organoids from tumor-bearing WT or Rnf5 −/− mice ( n = 3). Scale bar = 100μm. Graph shows percentage of cleaved caspase-3 + cells per immunostained organoid ( n = 12 fields). d Intracellular IFN-γ and TNF-α staining of p14 CD8 + T cells incubated for 72 h with 2 μg/ml GP33 peptide recognized by the TCR of P14 and bone marrow-derived dendritic cells (BMDCs) that were incubated with medium alone (no stimulation) or with conditioned medium (CM) from shControl or shRNF5 MODE-K cells. e Representative images (left) and quantification (right) of CD11c + cell immunostaining in the small intestine of WT or Rnf5 −/− mice on day 24 after injection of YUMM1.5 cells. Scale bar = 50μm ( n = 4). f Frequencies of total DCs and pDCs in Peyer’s patches from WT and Rnf5 −/− mice on day 10 after YUMM1.5 cell injection ( n = 6). g 10 days after tumor injection, DCs from GALT, dLN, and ndLN were isolated, pooled per group ( n = 10 mice/group), and were incubated with OT-1 CD8 + T cells stimulated with 2 μg/ml <t>OVA</t> peptide <t>(SINFEKL).</t> Intracellular IFN-γ and TNF-α of OT-1 CD8 + T cells were detected. Data are representative of three independent experiments ( b , d ) and two independent experiments ( a , c , e , f , g ) ≥3 mice per group. Graphs show the mean ± s.e.m. * P < 0.05, ** P < 0.005, *** P < 0.001, **** P < 0.0001 by two-tailed t test or Mann–Whitney U test ( a , b , c , e , f ) or one-way ANOVA with Tukey’s ( d ) correction for multiple comparisons
Biotin Labeled Ot (Anaspec Inc), supplied by AnaSpec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biotin-labeled ot (anaspec inc)/product/AnaSpec
Average 90 stars, based on 1 article reviews
biotin-labeled ot (anaspec inc) - by Bioz Stars, 2026-02
90/100 stars
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ova peptide specific for the tcr expressed by ot-ii t cells  (AnaSpec)
90
AnaSpec ova peptide specific for the tcr expressed by ot-ii t cells
IEC of Rnf5 −/− mice activate immune response and change anti-microbial peptide (AMP) expression. a Villi length and crypt depth calculated from H&E-stained sections of intestines from WT or Rnf5 −/− mice (WT, n = 30; Rnf5 −/− , n = 32). b qRT-PCR analysis of AMPs mRNA levels in IECs from small intestine of naive WT or Rnf5 −/− mice ( n = 6). c Representative images (left) and quantification (right) of cleaved caspase-3 immunostained small intestine organoids from tumor-bearing WT or Rnf5 −/− mice ( n = 3). Scale bar = 100μm. Graph shows percentage of cleaved caspase-3 + cells per immunostained organoid ( n = 12 fields). d Intracellular IFN-γ and TNF-α staining of p14 CD8 + T cells incubated for 72 h with 2 μg/ml GP33 peptide recognized by the TCR of P14 and bone marrow-derived dendritic cells (BMDCs) that were incubated with medium alone (no stimulation) or with conditioned medium (CM) from shControl or shRNF5 MODE-K cells. e Representative images (left) and quantification (right) of CD11c + cell immunostaining in the small intestine of WT or Rnf5 −/− mice on day 24 after injection of YUMM1.5 cells. Scale bar = 50μm ( n = 4). f Frequencies of total DCs and pDCs in Peyer’s patches from WT and Rnf5 −/− mice on day 10 after YUMM1.5 cell injection ( n = 6). g 10 days after tumor injection, DCs from GALT, dLN, and ndLN were isolated, pooled per group ( n = 10 mice/group), and were incubated with OT-1 CD8 + T cells stimulated with 2 μg/ml <t>OVA</t> peptide <t>(SINFEKL).</t> Intracellular IFN-γ and TNF-α of OT-1 CD8 + T cells were detected. Data are representative of three independent experiments ( b , d ) and two independent experiments ( a , c , e , f , g ) ≥3 mice per group. Graphs show the mean ± s.e.m. * P < 0.05, ** P < 0.005, *** P < 0.001, **** P < 0.0001 by two-tailed t test or Mann–Whitney U test ( a , b , c , e , f ) or one-way ANOVA with Tukey’s ( d ) correction for multiple comparisons
Ova Peptide Specific For The Tcr Expressed By Ot Ii T Cells, supplied by AnaSpec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ova peptide specific for the tcr expressed by ot-ii t cells/product/AnaSpec
Average 90 stars, based on 1 article reviews
ova peptide specific for the tcr expressed by ot-ii t cells - by Bioz Stars, 2026-02
90/100 stars
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ot anaspec #24275  (AnaSpec)
90
AnaSpec ot anaspec #24275
IEC of Rnf5 −/− mice activate immune response and change anti-microbial peptide (AMP) expression. a Villi length and crypt depth calculated from H&E-stained sections of intestines from WT or Rnf5 −/− mice (WT, n = 30; Rnf5 −/− , n = 32). b qRT-PCR analysis of AMPs mRNA levels in IECs from small intestine of naive WT or Rnf5 −/− mice ( n = 6). c Representative images (left) and quantification (right) of cleaved caspase-3 immunostained small intestine organoids from tumor-bearing WT or Rnf5 −/− mice ( n = 3). Scale bar = 100μm. Graph shows percentage of cleaved caspase-3 + cells per immunostained organoid ( n = 12 fields). d Intracellular IFN-γ and TNF-α staining of p14 CD8 + T cells incubated for 72 h with 2 μg/ml GP33 peptide recognized by the TCR of P14 and bone marrow-derived dendritic cells (BMDCs) that were incubated with medium alone (no stimulation) or with conditioned medium (CM) from shControl or shRNF5 MODE-K cells. e Representative images (left) and quantification (right) of CD11c + cell immunostaining in the small intestine of WT or Rnf5 −/− mice on day 24 after injection of YUMM1.5 cells. Scale bar = 50μm ( n = 4). f Frequencies of total DCs and pDCs in Peyer’s patches from WT and Rnf5 −/− mice on day 10 after YUMM1.5 cell injection ( n = 6). g 10 days after tumor injection, DCs from GALT, dLN, and ndLN were isolated, pooled per group ( n = 10 mice/group), and were incubated with OT-1 CD8 + T cells stimulated with 2 μg/ml <t>OVA</t> peptide <t>(SINFEKL).</t> Intracellular IFN-γ and TNF-α of OT-1 CD8 + T cells were detected. Data are representative of three independent experiments ( b , d ) and two independent experiments ( a , c , e , f , g ) ≥3 mice per group. Graphs show the mean ± s.e.m. * P < 0.05, ** P < 0.005, *** P < 0.001, **** P < 0.0001 by two-tailed t test or Mann–Whitney U test ( a , b , c , e , f ) or one-way ANOVA with Tukey’s ( d ) correction for multiple comparisons
Ot Anaspec #24275, supplied by AnaSpec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ot anaspec #24275/product/AnaSpec
Average 90 stars, based on 1 article reviews
ot anaspec #24275 - by Bioz Stars, 2026-02
90/100 stars
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Image Search Results


Potency of Leu 8 -OT and Pro 8 -OT at inducing calcium mobilization in mOTR and hOTR CHO cells

Journal: Molecular Pharmacology

Article Title: A Comparison of the Ability of Leu 8 - and Pro 8 -Oxytocin to Regulate Intracellular Ca 2+ and Ca 2+ -Activated K + Channels at Human and Marmoset Oxytocin Receptors

doi: 10.1124/mol.118.114744

Figure Lengend Snippet: Potency of Leu 8 -OT and Pro 8 -OT at inducing calcium mobilization in mOTR and hOTR CHO cells

Article Snippet: Leu 8 -OT (66-0-52; American Peptide Company) and Pro 8 -OT (58863; Anaspec) were reconstituted in dimethylsulfoxide (DMSO) (D4540; Sigma-Aldrich).

Techniques:

Potency of Leu 8 -OT and Pro 8 -OT at inducing membrane hyperpolarization in mOTR and hOTR CHO cells

Journal: Molecular Pharmacology

Article Title: A Comparison of the Ability of Leu 8 - and Pro 8 -Oxytocin to Regulate Intracellular Ca 2+ and Ca 2+ -Activated K + Channels at Human and Marmoset Oxytocin Receptors

doi: 10.1124/mol.118.114744

Figure Lengend Snippet: Potency of Leu 8 -OT and Pro 8 -OT at inducing membrane hyperpolarization in mOTR and hOTR CHO cells

Article Snippet: Leu 8 -OT (66-0-52; American Peptide Company) and Pro 8 -OT (58863; Anaspec) were reconstituted in dimethylsulfoxide (DMSO) (D4540; Sigma-Aldrich).

Techniques:

Representative primate species OTRs

Journal: The Journal of Pharmacology and Experimental Therapeutics

Article Title: Binding Characteristics of Two Oxytocin Variants and Vasopressin at Oxytocin Receptors from Four Primate Species with Different Social Behavior Patterns

doi: 10.1124/jpet.118.250852

Figure Lengend Snippet: Representative primate species OTRs

Article Snippet: Then 50 µ l of roughly 50,000 cpm ice-cold 125 I-OVTA was added in triplicate (technical replicates) to all wells in the presence or absence of 10 −11 to 10 −5 M Pro 8 -OT (CYIQNCPPG-NH2; Anaspec, Fremont, CA), Leu 8 -OT (CYIQNCPLG-NH2; Anaspec), or AVP (CYFQNCPRG-NH2; Anaspec) and incubated for 3 hours on ice.

Techniques:

IEC of Rnf5 −/− mice activate immune response and change anti-microbial peptide (AMP) expression. a Villi length and crypt depth calculated from H&E-stained sections of intestines from WT or Rnf5 −/− mice (WT, n = 30; Rnf5 −/− , n = 32). b qRT-PCR analysis of AMPs mRNA levels in IECs from small intestine of naive WT or Rnf5 −/− mice ( n = 6). c Representative images (left) and quantification (right) of cleaved caspase-3 immunostained small intestine organoids from tumor-bearing WT or Rnf5 −/− mice ( n = 3). Scale bar = 100μm. Graph shows percentage of cleaved caspase-3 + cells per immunostained organoid ( n = 12 fields). d Intracellular IFN-γ and TNF-α staining of p14 CD8 + T cells incubated for 72 h with 2 μg/ml GP33 peptide recognized by the TCR of P14 and bone marrow-derived dendritic cells (BMDCs) that were incubated with medium alone (no stimulation) or with conditioned medium (CM) from shControl or shRNF5 MODE-K cells. e Representative images (left) and quantification (right) of CD11c + cell immunostaining in the small intestine of WT or Rnf5 −/− mice on day 24 after injection of YUMM1.5 cells. Scale bar = 50μm ( n = 4). f Frequencies of total DCs and pDCs in Peyer’s patches from WT and Rnf5 −/− mice on day 10 after YUMM1.5 cell injection ( n = 6). g 10 days after tumor injection, DCs from GALT, dLN, and ndLN were isolated, pooled per group ( n = 10 mice/group), and were incubated with OT-1 CD8 + T cells stimulated with 2 μg/ml OVA peptide (SINFEKL). Intracellular IFN-γ and TNF-α of OT-1 CD8 + T cells were detected. Data are representative of three independent experiments ( b , d ) and two independent experiments ( a , c , e , f , g ) ≥3 mice per group. Graphs show the mean ± s.e.m. * P < 0.05, ** P < 0.005, *** P < 0.001, **** P < 0.0001 by two-tailed t test or Mann–Whitney U test ( a , b , c , e , f ) or one-way ANOVA with Tukey’s ( d ) correction for multiple comparisons

Journal: Nature Communications

Article Title: Gut microbiota dependent anti-tumor immunity restricts melanoma growth in Rnf5 −/ − mice

doi: 10.1038/s41467-019-09525-y

Figure Lengend Snippet: IEC of Rnf5 −/− mice activate immune response and change anti-microbial peptide (AMP) expression. a Villi length and crypt depth calculated from H&E-stained sections of intestines from WT or Rnf5 −/− mice (WT, n = 30; Rnf5 −/− , n = 32). b qRT-PCR analysis of AMPs mRNA levels in IECs from small intestine of naive WT or Rnf5 −/− mice ( n = 6). c Representative images (left) and quantification (right) of cleaved caspase-3 immunostained small intestine organoids from tumor-bearing WT or Rnf5 −/− mice ( n = 3). Scale bar = 100μm. Graph shows percentage of cleaved caspase-3 + cells per immunostained organoid ( n = 12 fields). d Intracellular IFN-γ and TNF-α staining of p14 CD8 + T cells incubated for 72 h with 2 μg/ml GP33 peptide recognized by the TCR of P14 and bone marrow-derived dendritic cells (BMDCs) that were incubated with medium alone (no stimulation) or with conditioned medium (CM) from shControl or shRNF5 MODE-K cells. e Representative images (left) and quantification (right) of CD11c + cell immunostaining in the small intestine of WT or Rnf5 −/− mice on day 24 after injection of YUMM1.5 cells. Scale bar = 50μm ( n = 4). f Frequencies of total DCs and pDCs in Peyer’s patches from WT and Rnf5 −/− mice on day 10 after YUMM1.5 cell injection ( n = 6). g 10 days after tumor injection, DCs from GALT, dLN, and ndLN were isolated, pooled per group ( n = 10 mice/group), and were incubated with OT-1 CD8 + T cells stimulated with 2 μg/ml OVA peptide (SINFEKL). Intracellular IFN-γ and TNF-α of OT-1 CD8 + T cells were detected. Data are representative of three independent experiments ( b , d ) and two independent experiments ( a , c , e , f , g ) ≥3 mice per group. Graphs show the mean ± s.e.m. * P < 0.05, ** P < 0.005, *** P < 0.001, **** P < 0.0001 by two-tailed t test or Mann–Whitney U test ( a , b , c , e , f ) or one-way ANOVA with Tukey’s ( d ) correction for multiple comparisons

Article Snippet: Cells were then mixed 1:1 with the BMDCs and incubated for 72 h pulsed with 2 μg/ml of OVA peptide (SINFEKL) (AnaSpec) or GP33 peptide (AnaSpec).

Techniques: Expressing, Staining, Quantitative RT-PCR, Incubation, Derivative Assay, Immunostaining, Injection, Isolation, Two Tailed Test, MANN-WHITNEY